Virginia Commonwealth University  ·  Dept. of Neuroscience

NeuroCrow Lab

Neuroscience of Chronic Pain

We investigate how nerve injuries trigger plasticity changes in spinal cord dorsal horn circuits — bridging molecular, circuit, and behavioral levels of analysis to develop targeted therapies for neuropathic pain.

Explore our research →
Research

The neuroscience
of neuropathic pain

Chronic pain affects millions of people and costs hundreds of billions of dollars each year in the United States. Our ability to treat neuropathic pain remains severely limited compared to the burden of this disease.

The Crowther Lab investigates how nerve injuries trigger plasticity changes in spinal cord dorsal horn circuits — the first area of the central nervous system to process somatosensory information. We use cutting-edge techniques including in vivo calcium imaging, viral circuit tracing, behavioral recording, and transgenic mouse models to understand how descending brainstem circuits that normally inhibit pain become dysfunctional after injury.

Our current research focuses on characterizing time-dependent changes in the locus coeruleus–spinal noradrenergic pathway, comparing oxaliplatin-induced peripheral neuropathy with traumatic nerve injury to identify rescuable intervention windows for endogenous analgesia systems.

01 ——
Descending Pain Control Circuits
Defining when and how top-down regulation from the locus coeruleus–spinal noradrenergic pathway fails after injury, and whether mechanisms differ between chemotherapy-induced and traumatic nerve injury models.
02 ——
In Vivo Spinal Cord Imaging
Using longitudinal in vivo calcium imaging of genetically identified dorsal horn projection neurons to track progressive changes in circuit function in both anesthetized and awake behaving mice — a technique pioneered in the Basbaum lab at UCSF.
03 ——
Oxaliplatin-Induced Neuropathy
Characterizing the trajectory of LC-spinal α2-adrenergic receptor pathway dysfunction in a chemotherapy-induced peripheral neuropathy model, which produces bilateral, distal-predominant sensory deficits distinct from focal nerve injury.
04 ——
Therapeutic Intervention Windows
Identifying time points where endogenous analgesic systems remain rescuable and providing mechanistic insight into why chemotherapy-induced neuropathy may respond differently to noradrenergic therapeutics than traumatic nerve injury.
Publications

Selected works

2025
Keratinocyte-TRPV1 sensory neuron interactions in a controllable mouse model of neuropathic itch
Crowther AJ*, Kashem S*, Jewell M, Chang H, Casillas MR, Midavaine É, Rodriguez S, Braz J, Kania A, Basbaum A.
PNAS
Article
2024
Long-term optical imaging of the spinal cord in awake, behaving mice
Ahanonu B*, Crowther AJ*, Kania A, Casillas MR, Basbaum A.
Nature Methods
Article
2024
Neuroanatomy of the Nociceptive System: From Nociceptors to Brain Networks
Motzkin J, Basbaum A, Crowther AJ.
International Review of Neurobiology · Neuropathic Pain, Vol. 179
Review
2024
Sex-specific expression of distinct serotonin receptors mediates stress vulnerability of adult hippocampal neural stem cells in mice
Luo YJ*, Bao H*, Crowther AJ*, Li YD, Chen ZK, Tart D, Asrican B, Zhang L, Song J.
Cell Reports
Article
2024
Anterior cingulate cortex projections to the dorsal medial striatum underlie insomnia associated with chronic pain
Li YD, Luo YJ, Su WK, Ge J, Crowther AJ, et al.
Neuron
Article
2018
An adeno-associated virus type 4-based toolkit for preferential targeting and manipulating quiescent neural stem cells in the adult hippocampus
Crowther AJ*, Lim S, Asrican B, Albright B, Wooten J, Yeh C, Bao H, Hu J, Asokan A, Song J.
Stem Cell Reports
Article
Team

The people behind
the work

AC
Andrew Crowther, PhD
Principal Investigator
Dr. Crowther completed his PhD at the University of North Carolina at Chapel Hill and postdoctoral training at UCSF in Allan Basbaum's lab, where he co-developed groundbreaking methods for long-term spinal cord optical imaging in awake, behaving mice. His lab investigates how descending brainstem circuits that normally inhibit pain become dysfunctional after nerve injury, using in vivo calcium imaging, viral circuit tracing, and transgenic mouse models.
Virginia Commonwealth University · Dept. of Neuroscience & Anatomy
VB
Vicki Brings, PhD
Research Scientist
Dr. Brings brings deep expertise in pain neurobiology to the Crowther Lab. Her work focuses on neuro-immune mechanisms and translational models of neuropathic pain, and she contributes to the lab's behavioral pharmacology and oxaliplatin-induced neuropathy research programs.
Crowther Lab · VCU
JV
Julian Vaughn
Graduate Student
Julian is a first year BDSP student performing his third rotation in the Crowther Lab.
Crowther Lab · VCU
News & Updates

What's happening
in the lab

New Publication
June 2025
Paper published in PNAS
Crowther AJ et al. publish on keratinocyte-TRPV1 sensory neuron interactions in a controllable mouse model of neuropathic itch, in collaboration with the Basbaum lab at UCSF.
Talk
April 2025
Seminar at VCU Dept. of Neuroscience & Anatomy
Dr. Crowther presented at the Department of Neuroscience and Anatomy seminar series at Virginia Commonwealth University.
Talk
March 2025
Invited speaker at NC State College of Veterinary Medicine
Dr. Crowther presented at the Molecular and Biomedical Sciences seminar series at North Carolina State University, Raleigh, NC.
New Publication
November 2024
Spinal cord imaging paper in Nature Methods
Ahanonu & Crowther et al. publish a landmark methods paper describing long-term optical imaging of the spinal cord in awake, behaving mice — enabling longitudinal single-neuron tracking across injury models.
Conference
October 2024
Poster at SFN Annual Meeting, Chicago
Dr. Crowther presented new findings at Neuroscience 2024, the annual meeting of the Society for Neuroscience.
Lab Update
2025
Crowther Lab opens at VCU
Dr. Crowther joins Virginia Commonwealth University as Assistant Professor in the Department of Neuroscience and Anatomy. The lab is recruiting graduate students passionate about pain neurobiology — reach out at andrew.crowther@vcuhealth.org.
Contact

Get in touch

We welcome inquiries from prospective graduate students, postdocs, and collaborators interested in pain neuroscience, spinal cord circuit imaging, or neuropathic pain. If you are passionate about neuroscience and find the field of chronic pain research exciting, reach out.

Principal Investigator
Andrew Crowther, PhD
Email
andrew.crowther@vcuhealth.org
Institution
Virginia Commonwealth University
Richmond, Virginia
Department
Neuroscience & Anatomy · School of Medicine
Office
9-005 Sanger Hall, MCV Campus